Mercedes driver Nico Rosberg has made it two wins from two starts to kick off 2016, with victory in the Bahrain Grand Prix.
Hamilton claimed pole on Saturday, but it was teammate Nico Rosberg who took the lead after Hamilton tangled with Valterri Bottas at the first corner and never looked back.
Ferrari's Kimi Raikkonen finished second, while Hamilton recovered to finish third.
Australian Daniel Ricciardo came in fourth for the second race in a row.
Rosberg now has a 17-point lead over teammate Hamilton in the battle for the championship after just two races.
FULL RACE RESULTS (57 laps)
1. Nico Rosberg, Germany, Mercedes, 57 laps, 1:33:34.696.
2. Kimi Raikkonen, Finland, Ferrari, 57, 1:33:44.978.
3. Lewis Hamilton, England, Mercedes, 57, 1:34:04.844.
4. Daniel Ricciardo, Australia, Red Bull, 57, 1:34:37.190.
5. Romain Grosjean, France, Haas, 57, 1:34:52.995.
6. Max Verstappen, Netherlands, Toro Rosso, 57, 1:34:55.625.
7. Daniil Kvyat, Russia, Red Bull, 56, +1 lap.
8. Felipe Massa, Brazil, Williams, 56, +1 lap.
9. Valtteri Bottas, Finland, Williams, 56, +1 lap.
10. Stoffel Vandoorne, Belgium, McLaren, 56, +1 lap.
11. Kevin Magnussen, Denmark, Renault, 56, +1 lap.
12. Marcus Ericsson, Sweden, Sauber, 56, +1 lap.
13. Pascal Wehrlein, Germany, Manor, 56, +1 lap.
14. Felipe Nasr, Brazil, Sauber, 56, +1 lap.
15. Nico Hulkenberg, Germany, Force India, 56, +1 lap.
16. Sergio Perez, Mexico, Force India, 56, +1 lap.
17. Rio Haryanto, Indonesia, Manor, 56, +1 lap.
DNF Carlos Sainz Jr., Spain, Toro Rosso, 29
DNF Esteban Gutierrez, Mexico, Haas, 9
DNF Jenson Button, England, McLaren, 6
DNS Sebastian Vettel, Germany, Ferrari, 0
DNS Jolyon Palmer, England, Renault, 0
